Figure 6. Influence of Rgg regulators on biofilm production.

SHPs can enhance biofilm biogenesis via the Rgg2/3 regulatory circuit and counter biofilm disassembly promoted by RopB. Crystal violet staining was performed to measure biofilm biomass of NZ131 derivatives grown 48 hours in 24-well polystyrene plates with (dark gray bars) or without (light gray bars) the addition of 50 nM sSHP3-C8. Levels of biofilm production were normalized to that of untreated NZ131 (wild type). Error bars indicate standard error from a minimum of three independent experiments.