Table 2. Plasmids used in this study.
| Plasmid | Description | Source |
| p7INT | Shuttle-suicide vector that integrates at streptococcal bacteriophage T12 attB site; ErmR | [50] |
| pBL111 | DNA fragment containing shp2 promoter region (500 bp) fused to luxAB by PCR and cloned into p7INT | This study |
| pBL112 | pFED760 with DNA fragments flanking ropB fused by PCR to created unmarked deletion | This study |
| pCA102 | pET14b-based expression vector with rgg3 cloned downstream of His-6 and SUMO tags; AmpR | [92]; this study |
| pCN58 | Shuttle plasmid containing promoterless Vibrio fischeri luxAB genes; AmpR, ErmR | [90] |
| pEep | pLZ12-Sp with eep cloned under synthetic promoter from pEVP3 | This study |
| pEVP3 | Plasmid encoding synthetic promoter and cat chloramphenicol resistance cassette; CmR | [88] |
| pFED760 | Shuttle vector pGh9-ISS1 deleted for ISS1 element; temperature-sensitive, ErmR | [21], [95] |
| pJC159 | pFED760 with ermB replaced by cat; CmR | This study |
| pJC175 | pLA101 with rgg3 replaced by cat cassette | This study |
| pJC178 | pLA101 with rgg3-shp3 replaced by cat cassette | This study |
| pJC183 | pFED760 with DNA fragments flanking eep fused by PCR to create unmarked deletion | This study |
| pJC186 | pJC159 with DNA fragments flanking rgg2 fused by PCR to create unmarked deletion | This study |
| pJC187 | DNA fragment containing shp3-aroE.2 promoter region (524 bp) fused to luxAB by PCR and cloned into p7INT | This study |
| pJC191 | pJC159 with DNA fragments flanking oppD fused by PCR to create unmarked deletion | This study |
| pJC205 | pJC187 with shp3 start codon mutated to GGG | This study |
| pLA101 | Fragment encompassing rgg3 and flanking DNA cloned into pFED760 | This study |
| pLZ12-Sp | Shuttle vector encoding spectinomycin resistance; pWV01 origin; SpR | [91] |
| pOppD | pLZ12-Sp with oppD cloned under synthetic promoter from pEVP3 | This study |
| pRgg2 | pLZ12-Sp with rgg2 cloned under its native promoter | This study |
| pRgg3 | pLZ12-Sp with rgg3 cloned under its native promoter | This study |
| pSHP3 | pLZ12-Sp with shp3 cloned under its native promoter | This study |
| pSHP31-18 | pSHP3 with shp3 C-terminal five amino acids deleted by inverse PCR | This study |
| pSHP31-20 | pSHP3 with shp3 C-terminal three amino acids deleted by inverse PCR | This study |
| pSHP31-22 | pSHP3 with shp3 C-terminal glycine deleted by inverse PCR | This study |
| pSHP315-23 | pSHP3 with shp3 N-terminal 14 amino acids deleted by inverse PCR | This study |
| pSHP317-23 | pSHP3 with shp3 N-terminal 16 amino acids deleted by inverse PCR; methionine added to peptide sequence to allow translation | This study |
Amp, ampicillin; Sp, spectinomycin.