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. 2011 Aug 4;7(8):e1002076. doi: 10.1371/journal.pcbi.1002076

Figure 1. Two colour fluorescence synapse patterns formed by a Jurkat T cell adhering to a lipid bilayer and a NK cell conjugating with a 221 cell.

Figure 1

A. T cell on a protein-rich supported bilayer loaded with ICAM1 (red) and CD58 (green), from Dustin et al. 1998. B. Intensity profile along transect shown in A (black), cell boundary at 5 and 25 microns. C. Surface reconstruction of fluorescence on a 221 cell transfected with HLA-Cw6-GFP (green) and ICAM1-Cherry (red). Contact interface limits (and free surface, on rear of cell) are indicated in white (magenta). D. Intensity profile along cell surface through contact interface in a mid range z-stack slice; the contact interface extends from approximately 2–9 microns. Bars show 5 microns.