Figure 1.

Chemical lysis of Arabidopsis cells. Three 7-day-old Arabidopsis seedlings were boiled for 10 minutes in the 100 μl lysis solution (100 mM Tris-HCl, pH 8.0, and indicated concentrations of SDS and NaOH). The solution was then mixed with 2× Laemmli sample buffer in 1:1 volume, and subjected to SDS-PAGE followed by CBB staining.