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. 2011 Aug 4;6(8):e22183. doi: 10.1371/journal.pone.0022183

Table 1. Yeast Strains.

Strain Genotype Source or reference
SLY101 MATα ade can1-100 cyh2r his3-11,15 leu2-3,112 trp1-1 ura3 [40]
YYO2 SLY101; p53-hsp82 [29]
SY1000 YYO2; leu2-3::PGAL1-p53-LEU2 [29]
SY1004 YYO2; leu2-3::PGAL1-p53Core Δ -LEU2 This study
BY4741 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Research Genetics
YDR007W BY4741; trp1Δ Research Genetics
K1000 YDR007W; pRS316 (URA3 CEN6), pRS314 (TRP1- CEN6) This study
K1001 YDR007W; Yp53 (PGAL1-p53 URA3 -CEN6), pRS314 This study
K1002 YDR007W; p53 R273H (PGAL1-p53 R273H TRP1-CEN6), pRS316 This study
K1003 YDR007W; p53 V143A (PGAL1-p53 V143A TRP1-CEN6), pRS316 This study
K1004 BY4741; pRS313 (HIS3 -CEN6), pRS315 (LEU2- CEN6) This study
K1005 BY4741; pRB16 (PADH1-p53 HIS3 -CEN6), pRS315 This study
K1006 BY4741; p53 R175H (PADH1-p53 R175H LEU2-CEN6), pRS313 This study
YGL043W BY4741; dst1Δ, pRS313, pRS315 Research Genetics
YGL043W1 YGL043W; pRB16, pRS315 This study
YGL043W2 YGL043W; p53 R175H, pRS313 This study
YDR315C BY4741; ipk1Δ Research Genetics
YDR315C1 BY4741; ipk1Δ, pRS316 This study
YDR315C2 BY4741; ipk1Δ, Yp53 This study
YDR315C3 BY4741; ipk1Δ, p53-1V143A (PGAL1-p53 V143A URA3-CEN6) This study
G1000 SLY101; pSCh202 (PGAL1-PHO5 URA3 CEN6), pRS314 This study
G1001 SLY101; pSCh212 (PGAL1-PHO5 URA3 CEN6), pRS314 This study
G1002 SLY101; pSCh202, Yp53 This study
G1003 SLY101; pSCh212, Yp53 This study
G1004 SLY101; pSCh202, Yp53V143A This study
DCY103 SLY101; dst1Δ [29]
D1000 DCY103; pSCh202, pRS314 This study
D1001 DCY103; pSCh212 , pRS314 This study
D1002 DCY103; pSCh202, Yp53 This study
D1003 DCY103; pSCh212, Yp53 This study
D1004 DCY103; pSCh202, Yp53 V143A This study
D1005 DCY103; pSCh212, Yp53 V143A This study