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. 2011 Aug 4;6(8):e22387. doi: 10.1371/journal.pone.0022387

Figure 1. ISL1 was highly expressed in adult pancreatic islets and could reduce apoptosis in HIT-T15 cells.

Figure 1

(A) Relative mRNA expression level of insulin in pancreatic islets from STZ mice (n = 6), Akita mice (n = 5) and db/db mice (n = 6) were measured by real-time RT-PCR. C57BL/6 mice (n = 6) were used as controls to STZ mice and Akita mice, db/w mice (n = 6) were as controls to db/db mice. Each bar represents mean ± SD (**p<0.01, *p<0.05, vs. the controls). (B) Level of ROS production was measured by flow cytometry analysis in HIT-T15 cells treated with glucose oxidase (GO) at various concentrations (0–100 mU/mL) for 4 h. (C) Level of apoptosis rate was measured by flow cytometry analysis in HIT-T15 treated with GO at various concentrations (0–100 mU/mL) for 4 h. (D) Relative level of ISL1 mRNA expression in HIT-T15 cells treated with different GO concentrations was examined by real-time RT-PCR. (E) Level of apoptosis rate was measured by flow cytometry analysis in stable ISL1 knockdown HIT-T15 cells treated with or without 5 mU/mL GO. Each bar represents mean ± SD from three samples (**p<0.01, vs. the control).