Table 1. Primers used in RT-PCR and real-time RT-PCR.
Primers | Primer sequences (5′–3′) | Product size (bp) |
18SRNA | F: GTAACCCGTTGAACCCCATT | 151 |
R: CCATCCAATCGGTAGTAGCG | ||
ISL1 | F: CTGCTTTTCAGCAACTGGTCA | 123 |
R: TAGGACTGGCTACCATGCTGT | ||
c-Myc | F: GCCACGTCTCCACACATCAG | 141 |
R: TCTTGGCAGCAGGATAGTCCTT | ||
CyclinD1 | F: GCGTACCCTGACACCCCTCTC | 183 |
R: CTCCTCTTCGCCTGATCC | ||
CyclinA | F: GCCTTCACCATTCATGTGGAT | 118 |
R: TTGCTGCGGGTAAAGAGACAG | ||
p53 | F: TTCCACCTGGGCTTCCTG | 144 |
R: GGATAGGTCGGCGGTTCAT | ||
Insulin | F: AGGACCCACAAGTGGAACAACT | 140 |
R: CAACGCCAAGGTCTGAAGGT |