Skip to main content
. Author manuscript; available in PMC: 2012 Aug 15.
Published in final edited form as: J Immunol. 2011 Jul 15;187(4):1970–1976. doi: 10.4049/jimmunol.1100841

Figure 3. GM-CSF fails to induce intratumoral sVEGFR-1 in mice with HIF-2α-deficient macrophages.

Figure 3

Mice with HIF-1α -or HIF-2α-deficient macrophages were treated with PBS or GM-CSF, as shown in Figure 1. At the time of sacrifice, tumor sections were excised and flash-frozen in liquid nitrogen. Frozen tissue was pulverized in liquid nitrogen and dissolved in TRIzol. Real-time PCR was performed for sVEGFR-1 (A) or VEGF (B). Results represent the mean ± SEM mRNA level as compared to the levels in PBS-treated control mice. Each group contains at least 15 mice.