. Author manuscript; available in PMC: 2012 Sep 1.

Published in final edited form as: J Surg Res. 2011 Mar 12;170(1):17–23. doi: 10.1016/j.jss.2011.02.018

Table 3.

Kinetic Parameters of Transporter-mediated Uptake

Method of Correction^*	Uncorrected	pH	Gly-Leu	Cold	Michaelis- Menten Equation
K_m (mM)	5.1 ± 1.4	0.7 ± 0.3	2.2 ± 0.6	2.4 ± 0.6	1.0 ± 0.8
K_m 95% CI	2.4-7.8	0.02-1.3	1.0-3.4	1.1-3.6	0.1-1.8
V_max (nmol/mg protein/10min)	41.4 ± 5.1	8.4 ± 0.9	19.6 ± 1.7	21.0 ± 1.8	12.2 ± 5.3
V_max 95% CI	31.2-51.5-	6.6-10.2	16.1-23.1	17.4-24.6	9.5-14.9
Kd (nmol\mg protein\10 min\ mM)					1.8 ± 0.5
Average % gly-sar transported by PepT1 (0.02-1 mM)		69	85	87	83

Kinetic characteristics of PepT1-mediated gly-sar uptake using different methods to correct total uptake for passive uptake (n=9). * $\overset{‒}{x} \pm SEM$ when appropriate