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. Author manuscript; available in PMC: 2012 Aug 15.
Published in final edited form as: J Immunol. 2011 Jul 15;187(4):1664–1673. doi: 10.4049/jimmunol.1100755

Figure 2. MyD88 expression is necessary for the enhanced XPA gene expression and survival after exposure to UVL in bone marrow derived cells.

Figure 2

(A) Macrophage cell lines derived from MyD88+/+ (wild type) mice or MyD88−/− (gene targeted) mice were incubated with medium alone, or medium containing the indicated doses of imiquimod, and XPA gene expression was assayed 6 hours later. (B) Bone marrow derived cells from MyD88+/+ (wild type) mice were incubated with the indicated doses of imiquimod for 24 hours, and then exposed to increasing doses of UVL, and survival was measured after another 24 hours using an MTT assay (see methods). (C) Bone marrow derived cells from MyD88−/− (gene targeted) mice were incubated with the indicated doses of imiquimod for 24 hours, and then exposed to increasing doses of UVL, and survival was measured after another 24 hours using an MTT assay (see methods). (data depicted represent the mean +/− SD of six replicates from a single representative experiment that was repeated three times; * p < 0.05; ** p < 0.01; ***p < 0.001, ANOVA)