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. Author manuscript; available in PMC: 2012 Aug 15.
Published in final edited form as: J Immunol. 2011 Jul 15;187(4):1664–1673. doi: 10.4049/jimmunol.1100755

Figure 3. NF-kB inhibition blocks the ability of imiquimod to enhance XPA gene expression as well as enhancement of survival after UVL exposure.

Figure 3

(A) P388 cells were pulse incubated with medium alone, medium containing DMSO vehicle or medium with BAY 11-7085 at the indicated dose for 30 minutes. After washing, the cells were then incubated with the indicated dose of imiquimod for 4 hours, and then gene expression was measured using qPCR. (B) The same experiment was repeated as in (A), except that the cells were incubated with imiquimod for 48 hours; then the cells were lysed, nuclei were isolated, and western blot performed (see methods). (C) Densitometry scanning of western blot depicted in (B). (D) The same experiment as in (A) except that P388 were incubated with the indicated dose of imiquimod for 24 hours, then exposed to the indicated doses of UVL, and survival was measured after another 24 hours using an MTT assay (see methods). (data depicted represent the mean +/− SD; **p < 0.01, ANOVA).