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. Author manuscript; available in PMC: 2012 Aug 15.
Published in final edited form as: J Immunol. 2011 Jul 15;187(4):1664–1673. doi: 10.4049/jimmunol.1100755

Figure 4. Imiquimod enhances functional DNA repair activity in bone marrow derived cells as measured in a gene reactivation assay.

Figure 4

A firefly luciferase pGL4.1[luc2] containing construct was either unirradiated (positive control), or irradiated with the indicated doses of UVL, and electroporated into either (A) P388 cells; (B) XS52 cells; (C) Bone marrow derived cells from MyD88+/+ mice. These host cells had been incubated with medium alone, or medium containing the indicated doses of imiquimod. Forty eight hours after transfection, a lysate was prepared, and luciferase activity was measured using luminometry and a reference luciferase to normalize for transfection efficiency (pGL4.70[hRluc], renila luciferase). Data are expressed as a % of control (% of expression of the irradiated luciferase to that of the unirradiated luciferase construct). See methods for the formula utilized to calculate percent gene reactivation. (data depicted represent mean +/− SD; * p < 0.05; ** p < 0.01, ANOVA).