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. Author manuscript; available in PMC: 2012 Aug 15.
Published in final edited form as: J Immunol. 2011 Jul 15;187(4):1664–1673. doi: 10.4049/jimmunol.1100755

Figure 5. Imiquimod enhances functional DNA repair activity in bone marrow derived cells as measured in a DNA dot-blot assay.

Figure 5

P388 cells (A–D) or XS52 cells (E–F) were pre-treated with medium, or medium containing the indicated dose of imiquimod for twenty-four hours, and then irradiated with the 10 mJ/cm2 (A–B, E,F) or 25 mJ/cm2 (C,D) of UVB. After another twenty-four hours, DNA was extracted, denatured, and crosslinked onto a nylon membrane, and probed with a cyclobutane pyrimidine dimer specific monoclonal antibody. Antibody binding to the DNA was detected using a secondary antibody and chemiluminescence (See methods). DNA dot blots (A,C,E); densitometry scanning of DNA dot blots depicted as a line point graph (B,D,F).