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. Author manuscript; available in PMC: 2012 Aug 10.
Published in final edited form as: J Am Chem Soc. 2011 Jul 15;133(31):11912–11915. doi: 10.1021/ja204758c

Figure 1.

Figure 1

Confocal spectrum-scan micrographs of taxoid-pyrazoline labeled microtubules in live CHO cells. CHO cells were treated with 1 µM of taxoid-pyrazoline 1a (a, b) or 3a (d, e) for 30 min and the fluorescence spectrum-scan in the region of 428–608 nm (a, d) and the corresponding DIC images (b, e) were recorded with a Zeiss LSM 710 microscope; λex = 405 nm. The colors rendered in the fluorescence micrographs were λ-encoded (real color). Scale bar = 20 µm. The average intensities of the unsaturated fluorescence-labeled cytoskeleton areas in 10 selected cells were plotted to give the in-cell fluorescence spectrum of 1a (c) or 3c (f) along with the standard deviations.