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. 2011 Aug 4;6(8):e23406. doi: 10.1371/journal.pone.0023406

Figure 2. Over-expression of HSP82 negatively regulates TPE but not mating type silencing.

Figure 2

(Ai) ADE2 color phenotype of various strains i.e., HSP82 (wild type), Δhsp82, Δhsc82, Δsba1, Δsir2, over-expressing HSP82 (HSP82+pHSP82) and over-expressing HSP82 in Δsba1 background. Pink colored colony represents telomere silencing and white colored colony represents de-repression of telomere silencing. (Aii) YFR057w is silenced in all single mutants as well as in wild type strain. (Bi) Over-expression of HSP82 de-represses the expression of YFR057w. ACT1 serves as loading control. (Bii) Quantification of YFR057w transcript is done from 4 independent experiments in HSP82 overexpressed cells. The band intensity values are normalized against ACT1. Each bar represents mean density ± SD.