Figure 3. Single mutants as well as overexpression of HSP82 do not alter mating type silencing.

(A) Mating type silencing phenotype of different strains (as indicated) when mated with the tester strain YDS32 (MATα). Δsir2 acts as a negative control. (Bi) RT-PCR analysis of HMLα2 transcripts in different strain backgrounds (as indicated) shows that HMLα2 transcript levels are similar in HSP82 overexpressing cells and wild type cells, ACT1 serves as loading control. (Bii) Quantification of HMLα2 transcript from three independent experiments are normalized with ACT1 and plotted. Each bar represents mean density ± SD.