Figure 3. Differences in plaque size between Anc(P) and evolved Qβ.

Plaque size was analyzed using A/λ as an indicator strain on LB agar medium. Most of the plaques of Anc(P) were large but some small plaques were also found. Both large and small plaques reproduced heterogeneity in plaque size (data not shown). In the copropagation regime, the plaque size decreased with number of generations, and plaques of M163(P) became quite small (indicated with arrows), while the plaques of M165_2(P) were still heterogeneous. In the Qβ propagation regime, the plaque size of S94_3(P) became uniformly large.