Table 2. Fitness of ancestral and evolved Qβ.
| E. coli populations | ||||
| Qß populations | Anc(C) | M54(C) | M163(C) | M165_2(C) |
| Anc(P) | 4.384.20 | 0.410.41 | 0.400.35 | 0.340.21 |
| M54(P) | 4.174.11 | 0.880.79 | 0.890.85 | 0.680.59 |
| M163(P) | 3.543.46 | 0.930.76 | 1.120.98 | NA |
| M165_2(P) | 3.743.43 | 0.480.32 | NA | 0.830.80 |
| S94_3(P) | 4.884.76 | NA | NA | NA |
Each experiment was conducted in duplicate and the two values are shown. The fitness of Qβ was calculated as (Fitness) = Log10 (PFU/ml_max/PFU/ml_0h), where PFU/ml_max is PFU/ml of free phage in which we adopted the higher value between the values of 7 h or 22–29.5 h after infection, and PFU/ml_0h is PFU/ml of free phage just after infection. The growth curves are shown in Figure S1. NA, not available.