Fig. 2.
In vivo tumor treatment experiments. C57BL/6 mice (n = 5 per group) were initially challenged with TC-1 tumor cells (1 × 105/mouse) by s.c. injection. Mice were injected with DNA (100 μg/mouse) with or without added α-GalCer (2 μg per injection) three days after tumor challenge. The mice were vaccinated 1 week later with various vaccine combination regimens, and were monitored twice weekly for evidence of tumor growth by inspection and palpation. Tumor volumes were measured starting at day 7 after tumor challenge. (A) Line graph depicting the tumor volumes in mice of different tumor treatments (means ± SD). (B) Kaplan–Meier survival analysis of tumor treatment experiments in mice. Data shown are from one of two representative experiments.