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. Author manuscript; available in PMC: 2012 Jul 8.
Published in final edited form as: Cell. 2011 Jul 8;146(1):119–133. doi: 10.1016/j.cell.2011.06.026

Figure 2. Autosomal transgenes attract Xist RNA way from Xi.

Figure 2

A. Map of Xist, FISH probes, and transgenes. P, PasI.

B. Serial Xist RNA/Tg DNA FISH in X-RA cells ± dox. Tg, transgene insertion site. Endogenous Xist, RA probe (RA); transgenic Xist, 5-Xist-riboprobe mix (5mix). Arrows, trans-migrated endogenous Xist to transgene site.

C. H3K27me3 immunostaining followed by DNA FISH in X-RA cells.

D. RNA FISH of female X+PE1 cells using probes E1 and E7 ± dox. Arrows, trans-migration of endogenous Xist to transgene site.

E. qRT-PCR for total (uRA) and endogenous (dRE) Xist.