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. Author manuscript; available in PMC: 2012 Jul 8.
Published in final edited form as: Cell. 2011 Jul 8;146(1):119–133. doi: 10.1016/j.cell.2011.06.026

Figure 3. YY1 is required for Xist localization.

Figure 3

A. Map of the proximal 2-kb region of Xist. One CTCF and three putative YY1 binding sites near Repeat F are shown.

B. Western blot, qRT-PCR, and combined Xist RNA FISH/CTCF immunostain 48 hours after Ctcf knockdown using C1 or C3 siRNA. Averages ± SD of three independent experiments shown.

C. YY1 Western blot and Yy1/Xist qRT-PCR after Yy1 knockdown using Y1 or Y2 siRNA. Averages ± SD from 7 independent experiments shown for qRT-PCR. One representative Western blot shown.

D. Xist FISH after Yy1 knockdown. Cells with pinpoint (arrows) or no Xist were scored negative. Averages ± SD from 206–510 nuclei/sample from three independent experiments.

E. H3K27me3 immunostaining (blue) followed by Xist RNA FISH in Yy1-knockdown cells. Two representative patterns shown. Histogram shows counts (n=62–138).