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. 1967 Aug;94(2):409–414. doi: 10.1128/jb.94.2.409-414.1967

Effect of Adenosine Monophosphate, Adenosine Diphosphate, and Reduced Nicotinamide Adenine Dinucleotide on Adenosine Triphosphate-dependent Carbon Dioxide Fixation in the Autotroph Thiobacillus neapolitanus1

Jerry V Mayeux a,2, Emmett J Johnson a,3
PMCID: PMC315055  PMID: 4292312

Abstract

The observation that adenosine triphosphate (ATP)-dependent CO2 fixation in extracts of chemosynthetic and photosynthetic autotrophs may be regulated in part by adenosine monophosphate (AMP) was extended to the strict autotroph Thiobacillus neapolitanus (X). In addition, this report presents data which include adenosine diphosphate (ADP) in the regulatory role. When the primary CO2 acceptor, ribose-5-phosphate, was replaced by ribulose-1,5-diphosphate, no inhibition of CO2 fixation occurred unless the Mg++ concentration was limiting. A molar ratio of 5:1 AMP or ADP to ATP reduced the specific activity (micromoles of CO2 fixed per milligram of protein per minute) of the extracts from 0.22 to 0.12 and 0.11, respectively. The reported stimulation of the carboxylative phase of ATP-dependent CO2 fixation by reduced nicotinamide adenine dinucleotide (NADH2) was investigated. Adding NADH2 to the extracts did not stimulate CO2 fixation, even at carbonate levels from 0.05 to 30 μmoles, except in the absence of ribose-5-phosphate. Slight increases in CO2 fixation were noted when the assay system was incubated in air instead of the usual helium atmosphere.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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