A, Representative whole-cell Kv4.3-WT plus KChIP2-WT (left), Kv4.3-L450F plus KChIP2-WT (middle) and Kv4.3-G600R plus KChIP2-WT (right) traces recorded in HEK293 cells elicited by step depolarization of 500 ms duration to +40 mV from a holding potential of −80 mV in 10 mV increments.
B, The current voltage relationship for WT (n= 18), L450F (n=15) and G600R (n=15) Kv4.3 channels co-expressed with KChIP2-WT. All values represent mean±SEM. * p<0.05 vs. Kv4.3-WT plus KChIP2-WT.
C. Bar graph showing peak current density at 40 mV for WT (n= 18), L450F (n=15) and G600R (n=15) Kv4.3 channels co-expressed with KChIP2-WT. * p<0.05 vs. Kv4.3-WT plus KChIP2-WT.