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. Author manuscript; available in PMC: 2011 Aug 4.
Published in final edited form as: Nature. 2010 Dec 15;469(7329):245–249. doi: 10.1038/nature09585

Figure 4. Aberrant joining in H2AX-deficient cells.

Figure 4

(a) PCR products representing normal and deleted (bracket) pMX-DELCJ CJs from WT:DELCJ, Artemis−/−:DELCJ and Artemis−/−:H2AX−/−:DELCJ abl pre-B cells treated with STI571. Serial five-fold dilutions of genomic DNA were amplified. IL2 gene PCR is a loading control. (b–c) (b) Base-pairs deleted and (c) microhomology utilization in pMX-DELCJ CJs sequenced from WT:DELCJ and Artemis−/−:H2AX−/−:DELCJ abl pre-B cells (Supplementary Fig. 14). The total number of sequenced coding joints (n) is indicated. Pie chart shows fraction of joints with 1, 2 or ≥ 3 microhomologies and total number (center) of joints using microhomologies. (d) Model for H2AX function in DNA end processing in G1-phase lymphocytes. Error bars represent standard error of the mean (SEM). p-values were determined using Student’s t-test with Welch’s correction for unequal variances.