| Time (min) | A% (99:1 H2O:MeCN) | B% (99:1 MeCN: H2O) | Flow Rate (mL/min) |
| 0.00 | 95.0 | 5.0 | 1.000 |
| 1.00 | 40.0 | 60.0 | 1.000 |
| 31.00 | 30.0 | 70.0 | 1.000 |
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