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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: J Orthop Res. 2011 Mar 29;29(10):1521–1527. doi: 10.1002/jor.21408

Fig. 3.

Fig. 3

Osteoclast formation is impaired in Raw264.7 cells with decreased SH3BP2 expression. (A) In non-infected cells, TRAP activity was increased by RANKL in a time-dependent manner. shRNA knockdown of SH3BP2 significantly decreased TRAP activity compared with control cells; TRAP activity was decreased to 29% of that with empty vector and 27% of that with scramble shRNA, *p<0.005 compared with both controls. (B) The number of TRAP+, multinucleated (MN) osteoclasts (OCs) derived from the cells infected with SH3BP2 shRNA was significantly reduced compared with controls; the number of TRAP+, multinucleated osteoclasts derived from Raw264.7 cells infected with SH3BP2 shRNA was 42% of that with empty vector and 44% of that with scramble shRNA, **p<0.001 compared with both controls.