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Sh3bp2(−/−) BMMs formed smaller osteoclasts with less TRAP staining and novel external cell membrane protrusions than wild-type BMMs. BMMs were treated with RANKL (100ng/ml) and m-CSF (30ng/ml) and osteoclast size, density of TRAP staining and osteoclast area (by F-actin staining) were analyzed and revealed significant differences.
