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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: J Orthop Res. 2011 Apr 14;29(10):1544–1553. doi: 10.1002/jor.21436

Figure 3.

Figure 3

Alkaline phosphatase staining of wells after 8 days of culture with MSCs alone (MSC) or MSCs and HSPCs in co-culture (CC) at specified seeding densities (400 or 1000 HSPCs seeded onto 40,000 MSCs) either with (+) (D–F) or without (−) (A–C) the addition of dexamethasone. The scale bar represents 200 µm for all microscopy images, save insets. Bottom-left insets (A–F) show the overall alkaline phosphatase staining of the wells, and top-right insets (B, C, E and F) show a 3× magnified view (with respect to the respective main images) of co-cultures, with arrows indicating apparent HSPCs.