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. 2011 May 4;100(9):2184–2192. doi: 10.1016/j.bpj.2011.03.031

Figure 6.

Figure 6

2D-BN/SDS-PAGE analysis of ATP synthase in Drosophila mitochondria including wild-type (WT), cardiolipin synthase mutant (ΔCLS), and tafazzin mutant (ΔTAZ). (A) BN-PAGE molecular weight markers. The molecular mass of markers is given in kDa. (B) 2D-BN/SDS-PAGE analysis of the α-subunit of ATP synthase using fluorescing secondary antibody from Li-Cor. In the WT, most of ATP synthase was recovered as dimer (D); in ΔTAZ about equal proportions of ATP synthase were recovered as dimer and as monomer (M); and in ΔCLS most of ATP synthase was recovered as monomer. (C) 2D-BN/SDS-PAGE analysis of the α-subunit of ATP synthase using horseradish peroxidase-conjugated secondary antibody. Some dimers remain present in the ΔCLS mutant.