Table 1.
ZFN-mediated engineering of endogenous genes in plants
| Plant | Target gene | ZFN delivery | ZFN development | Genome engineering | Reference |
|---|---|---|---|---|---|
| Tobacco | SurA and B | Electroporation | OPEN | NHEJ (gene knockout) | Maeder et al. (2009) |
| Tobacco | SurA and B | Electroporation | OPEN, modular assembly | HR (gene correction) | Townsend et al. (2009) |
| Zea mays | IPK1 | Whisker-mediated transformation | Two-finger modules | HR (gene knockout by selection marker addition) | Shukla et al. (2009) |
| Tobacco | CHN50 | Agrobacterium | Two-finger modules | HR (gene knockout by selection marker addition) | Cai et al. (2009) |
| Arabidopsis | ABI4, NHEJ | Agrobacterium | Modular assembly | NHEJ (gene knockout) | Osakabe et al. (2010) |
| Arabidopsis | ADH1, TT4 | Agrobacterium | OPEN | NHEJ (gene knockout) | Zhang et al. (2010) |