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. Author manuscript; available in PMC: 2011 Aug 5.
Published in final edited form as: Nature. 2010 Sep 16;467(7313):285–290. doi: 10.1038/nature09342

Figure 2. Differentiation of cell lines.

Figure 2

a, Hematopoietic colony number per 100,000 EB cells differentiated from indicated cell lines. b, Alizarin Red staining of osteogenic cultures of B-iPSC (left) and F-iPSC (right). Top: 3 cm culture dish; Bottom: stained colonies; scale bar, 500 μm. c, Quantification of elemental calcium by inductively coupled plasma – atomic emission spectroscopy49 in 5×105 cells after osteogenic differentiation of indicated cell lines. d, Q-PCR of osteogenic genes, Bglap, Sp7, and Runx2 in indicated cell lines after osteogenic differentiation. Gene expression was normalized to Actin. n=number of independent clones tested. Error bars=s.d.