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. Author manuscript; available in PMC: 2011 Aug 5.
Published in final edited form as: Am J Physiol Cell Physiol. 2007 Nov 21;294(1):C372–C379. doi: 10.1152/ajpcell.00186.2007

Fig. 4.

Fig. 4

Downregulation of cardiac Na+ channel mRNA by ANG II and H2O2. H9c2 or acutely isolated neonatal cardiomyocytes cultured in SFM or exposed to ANG II (100 nmol/l or 2 nmol/l), H2O2 (20 µmol/l or 40 nmol/l), or ANG II (100 nmol/l or 2 nmol/l) for 48 h. ANG II and H2O2 resulted in reduced Na+ channel mRNA abundance in both H9c2 (A) and neonatal cardiomyocytes (B). In both cases, the ANG II effect could be prevented by PEG-catalase (ANG II+CAT). Reduction in Na+ channel mRNA mediated by ANG II was prevented by the NADPH oxidase inhibitor apocynin (Apo) or the reactive oxygen species scavengers tiron or Tempol in H9c2 cells (C). *P < 0.05.