FIGURE 4.
Axon microelectrofusion demonstrated by the passage of soluble cytoplasmic green fluorescent protein (GFP) from one axon fusion partner into another. (a) Bright field image showing hippocampal axons in culture. Single arrow points to location where microelectrofusion was induced. (b) Prefusion image of the same field as in ‘a’ showing axons containing soluble cytoplasmic fluorescent GFP. (c) After microelectrofusion, GFP passed from the axon at the bottom left into an axon fusion partner that did not originally have GFP, indicating successful fusion of the two axonal compartments.