Table 1.

Analysis of X4-Cernunnos interactions by calorimetry

Cernunnos *	Xrcc4 *	Kd (μM)	Commentary	Reference
WT1–224	WT1–203	3.9 ± 0.2		29
WT1–224	WT1–157	4.1 ± 0.2	X4(1-157) central coiled-coil 60 Å shorter than X4(1–203)	this study
WT1–224	E55R1–157	no interaction	in helix α1, salt bridge with R64C	this study
WT1–224	D58R1–157	no interaction	in helix α1, close to E55C and R64C	this study
WT1–224	M61R1–157	no interaction	in loop α1–α2, in contact with P116C and L115C	this study
WT1–224	E62R1–157	3.8 ± 0.2	in helix α2, salt bridge with K65X	this study
WT1–224	K65E1–157	> 40	in helix α2, salt bridge with E111C and E62X	this study
WT1–224	E69R1–157	5.0 ± 0.2	in helix α2, salt bridge with K99X and hydrogen bond with Y84X	this study
WT1–224	F106E1–157	no interaction	in strand β7, in contact with L65C, A67C, F72C, and L112C	this study
WT1–224	F106A1–157	> 40	see above	this study
WT1–224	F106Y1–157	> 40	see above	this study
E111K1–224	WT1–157	6.9 ± 0.2	in strand β6, salt bridge with K65X	this study
R64E1–224	WT1–203	no interaction	in loop α2–α3, salt-bridge with E55X and close to D58X	29
L65D1–224	WT1–203	> 80	in loop α2–α3, in contact with F106X	29
L115D1-224	WT1–203	no interaction	in strand β7, in contact with F106XM61X, M59X and L108X	29

^*The limits of the constructs used for X4 and Cernunnos are indicated in superscript.