Fig. 4.

The N-terminal half of the SNARE motif of soluble synaptobrevin binds to DPC micelles but not to liposomes. (A–C) Superposition of ¹H-¹⁵N HSQC spectra of the 10 μM soluble synaptobrevin(1–96) fragment (black contours) alone or in the presence (red contours) of 300 mM DPC at pH 6.0 (A), POPC liposomes (50 mM lipids) at pH 6.0 (B), or POPC liposomes (50 mM lipids) at pH 7.0 (C). In B, cross-peaks that disappear upon addition of liposomes are labeled; cross-peaks from Arg side chains, which are observable at pH 6.0 but not at pH 7.0, are labeled Rsc. Note that in A there are less observable cross-peaks in DPC than in ref. 26 because we used a much lower protein concentration, but the data are fully consistent.