Fig. 1.

Surface plasmon resonance analysis of the interaction between FcγRIIIa and hIgG1 glycovariants. (A) Overlay of sensorgrams for binding of 125 nM FcγRIIIa variants to fucosylated (dotted lines) and afucosylated (continuous lines) IgG1s. The association phase is represented by a solid bar above the curves. (B) The N-linked carbohydrate moiety contains a core pentasaccharide (gray box), shared among high mannose, hybrid and complex-type oligosaccharides, and variable core fucosylation, GlcNAc bisection and/or composition of outer arms. GlcNAc, N-acetylglucosamine; Fuc, fucose; Man, mannose; kif, kifunensine.