Table 1.

Summary of affinity constants determined by equilibrium and kinetic analysis

Ligand(hIgG1)	Analyte(FcγRIIIa)	kon,1/Ms	koff,1/s	KD, Mkinetic	Steady state
Fucosylated	native	ND*			3.0E-07
Afucosylated	native	1.58E+06	4.6E-03	2.9E-09	7.2E-09
Fucosylated	−N45N162	ND*			4.9E-07
Afucosylated	−N45N162	1.01E+06	6.1E-03	6.0E-09	1.1E-08
Fucosylated	−N45N162 kif	ND*			2.8E-07
Afucosylated	−N45N162 kif	1.34E+06	3.0E-03	2.3E-09	5.6E-09

Representative data from a single experiment, which was repeated twice with similar results.

^*ND, not determined. Kinetics too fast for exact determination.