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. 2011 Jul 18;108(31):12827–12832. doi: 10.1073/pnas.1105774108

Fig. 5.

Fig. 5.

Recombinant ICOSL rescues the Tfh cell defect in NIK KO mice. (A) WT and NIK KO mice were immunized with SRBC on day 0 along with injection of a recombinant ICOSL-Fc fusion protein or a control Fc protein. The mice received three additional injections of ICOSL-Fc or control Fc at the indicated days postimmunization and then subjected to Tfh cell analyses. (B and C) The frequency of Tfh cells among CD4 T cells was quantified by flow cytometry and presented as a representative flow cytometry graph (B) and mean value of multiple mice (C). (D and E) Rag2 KO mice were adoptively transferred with a combination of WT T cells and NIK KO B cells. The recipient mice were immunized with SRBC along with injection with either control Fc or ICOSL-Fc, as described in A. The frequency of Tfh cells among CD4 T cells was quantified by flow cytometry and presented as a representative flow cytometry graph (D) and mean values (E).