Fig. 2.

Crosslinking of EspP β domain residues 1113 and 1214 to Skp, BamB, and BamD. AD202 cells were transformed with pDULEBpa and a derivative of pRI22 or pRI23 harboring an amber codon at residue 1113 (A) or 1214 (B). Cells were subjected to pulse-chase labeling at 25 °C or 37 °C after the addition of IPTG, and samples were processed as described in the legend of Fig. 1. The crystal structure of the β domain (25) and the location of each residue are shown in the diagram on the right.