Fig. 3.

Crosslinking of EspP β domain residue 1300 to BamA. AD202 cells were transformed with pDULEBpa and a derivative of pRI22 or pRI23 harboring an amber codon at residue 1300. Cells were subjected to pulse-chase labeling at 25 °C or 37 °C after the addition of IPTG, and samples were processed as described in the legend of Fig. 1. The crystal structure of the β domain and the location of residue 1300 are shown in the diagram on the right.