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Semiquantitative RT-PCR confirmed that GSPCs expressed p53. Total RNAs were extracted from both GSPC colonies and H9 colonies and reverse-transcribed into cDNA. Quantified cDNA (50 ng) was used for PCR with p53-specific primers. GSPC colonies expressed p53 at a level comparable to H9 colonies. MW, molecular weight 1-kb DNA ladder (Invitrogen).
