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. 2011 Jul 18;108(31):12675-12680. doi: 10.1073/pnas.1104030108

Fig. 5.

Fig. 5.

Validation of the processive model by input-output response. (A and B) Serum-starved HeLa cells were stimulated with the different concentration of EGF (blue, 0.01; cyan, 0.03; green, 0.1; yellow, 0.3; orange, 1.0; pink, 3.0; and red, 10( × 10-6 [g/L])) or 2.0 × 10-5 M U0126 (black) for 5 min, and then stained with anti-pTpY-ERK1/2 antibody. (A) Fluorescent signals in each cell are represented in a histogram, demonstrating unimodal distributions (n > 90 cells). (B) The average fluorescent intensities are plotted as a function of EGF concentration with SD. Hill coefficient value, nH, was obtained by curve fitting with standard Hill equation (black line). (C) The average of fraction of pTpY-ERK are plotted as a function of EGF concentration with SD (n = 5). This data is same as shown in Fig. 2G (red line). (D) The average of fraction of pTpY-ERK was plotted as a function of ratio value of phosphorylated MEK1/2 to total MEK1/2 with SD (n = 5). (E and F) The pTpY-ERK concentration at time = 10 (min) after stimulation in distributive (E) and processive (F) model are plotted as a function of phosphorylated MEK concentration.