Fig. 3.

Ligament cell attachment to COMP is independently inhibited by either the VV-containing peptide or an RGD peptide. Ligament cells were preincubated with carrier alone or inhibitory peptides (GRGDTP or GRADSP at 1 mg/ml; VV at 50 μ/ml) and were plated on wells coated with recombinant COMP or fibronectin (10 μg/ml or 1 μg/ml, respectively) as described in “Materials and methods” section. Dotted bars fibronectin, shaded bars COMP, background binding to 10 mg/ml BSA (- - -). Results are means ±2 × SEM; n ≥ 12. * P < 0.005, significant difference compared with inhibition of cell attachment by GRGDTP or VV alone