Fig. 4.

Ligament cell attachment to COMP is inhibited by function-blocking antibodies to CD47 and αVβ3 integrin. a Ligament cells were preincubated with different concentrations of CD47 antibody and plated on wells coated with 26.2 μg/ml (50 nM) COMP as described in “Materials and methods” section. Cells preincubated with normal mouse IgG (·−·); cells preincubated with CD47 antibody (filled diamonds); background binding to 10 mg/ml BSA (- - -). b Ligament cells were preincubated with carrier alone, function-blocking antibody or normal mouse IgG at 25 μ/ml and were plated on wells coated with recombinant COMP (26.2 μg/ml) as described previously. a, b Results are means ±2 × SEM; n ≥ 18. a, b * P < 0.005, significant difference comparing CD47 antibody inhibition with control untreated cells. b ^¶ P < 0.05, significant difference comparing αVβ3 antibody inhibition with control untreated cells