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. 2011 Jun 7;286(32):27902–27914. doi: 10.1074/jbc.M110.186221

FIGURE 7.

FIGURE 7.

SREBP-1c squelches recruitment of PDX-1 coactivators. A, strategy for Gal4-reporter gene assay. B, domain swap experiments. Gal4 DNA-binding domain (1–147 amino acids) is attached to the indicated PDX-1 proteins. Gal4-PDX-1 was cotransfected with pSV-β-gal and (Gal4)8-Luc in HIT or HepG2 cells. The (Gal4)8-Luc activity of the empty expression vector (pM) was set to 1.0. C and D indicated amounts of Gal4-PDX-1(1–283) or Gal4-PDX-1(1–149) and SREBP-1c (0–0.25 μg) were cotransfected with pSV-β-gal and (Gal4)8-Luc in HepG2 cells (n = 6). E, Gal4-PDX-1(1–283) (0.25 μg) or Gal4-PDX-1(1–149) (0.125 μg) and indicated amounts of DN-(Δ1–67)-SREBP-1c were cotransfected with pSV-β-gal and (Gal4)8-Luc in HepG2 cells (n = 3). F, Gal4-PDX-1(1–149) (0.125 μg), SREBP-1c, or DN-(Δ1–67)-SREBP-1c were cotransfected with pSV-β-gal and (Gal4)8-Luc in HIT cells (n = 3). Luciferase activity was normalized to the pSV-β-gal values. The (Gal4)8-Luc activity of the empty expression vector (pM) was set to 1.0 (C–F). Data are means ± S.E. (C and D).