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. 2011 Jun 10;286(32):27927–27935. doi: 10.1074/jbc.M111.255810

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Mitosis-specific reduced binding of eEF1A to its GEF is specific to eEF1A-eEF1D interaction. A fraction of total protein used for immunoprecipitation (input) and the anti-FLAG immunoprecipitates (IP Flag) from non-synchronized (NS) and mitotic (M) HeLa cells stably expressing FLAG-eEF1A (A), FLAG-eEF1B2 (B), or FLAG-eEF1G (C) were immunoblotted for the indicated proteins. Images represent one of three or four independent experiments. The intensity of bands from all experiments was quantified by densitometry. Quantitative data is represented as mean ± S.E.