FIGURE 7.

Kinetics and phospholipid headgroup specificity of Lnt. A, time-dependent formation of N-acyl-FSL-1 using PE (500 μm), PG (500 μm), and PA (100 μm) as acyl donors was analyzed by Tricine-SDS-PAGE and immunoblotting. Structures of the headgroups of PE, PA, and PG are displayed with R indicating the 1-palmitoyl-2-oleoyl-sn-glyceryl group. B, initial Lnt activities are displayed as function of the phospholipid concentration using PE (○), PA (▴), and PG (□). C, double-reciprocal plot showing initial Lnt activities with FSL-1 at various concentrations (1–5 μm) using either PE, PG, PC (500 μm, dashed line), or PA (100 μm) as acyl donor. Data points represent averages of three independent experiments.