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. 2011 Jun 21;286(32):27971–27979. doi: 10.1074/jbc.M111.224352

FIGURE 6.

FIGURE 6.

Knockdown of Crebh expression attenuates Cb1r signaling-mediated up-regulation of hepatic gluconeogenesis. A and B, primary rat hepatocytes were infected with adenovirus USi (50 multiplicity of infection) or CREBH shRNA (CREBHi, 50 multiplicity of infection) for 48 h followed by treatment with 2-AG for 12 h, and total RNA was isolated for qPCR analysis (A). Measurement of glucose production (B) was performed using glucose-free media supplemented with gluconeogenic substrate sodium lactate (20 mm) and sodium pyruvate (1 mm). Data represent mean ± S.D. of three individual experiments. *, p < 0.05; **, p < 0.001 versus vehicle and 2-AG treated cells in the absence of CREBHi overexpression, respectively. C, primary human hepatocytes were infected with adenovirus USi (50 multiplicity of infection) or CREBH shRNA (CREBHi, 50 multiplicity of infection) for 48 h followed by treatment with 2-AG for 12 h, and total RNA was isolated for qPCR analysis. Data represent mean ± S.D. of three individual experiments. *, p < 0.05; **, p < 0.001 versus vehicle and 2-AG treated cells in the absence of CREBHi overexpression, respectively.