FIGURE 7.

T1165-K13^−IL6 cells establish peritoneal plasmacytomas without pristane preconditioning and lead to disseminated disease involving visceral organs. A and B, BALB/cAnNCr mice were injected intraperitoneally with the indicated cells, and tumor growth was monitored by physical examination (A) or bioluminescence imagine (B) as described under “Materials and Methods.” C, plasmacytomas isolated at autopsy from mice injected with the T1165-Luc-K13^−IL6 cells. D, splenomegaly in mice injected with T1165-Luc-K13^−IL6 cells (right panel) as compared with a normal spleen in those injected with the T1165-Luc-vector cells (left panel). E, immunoblot analysis showing the presence of FLAG-tagged K13 in the parental T1165-Luc-K13^−IL6 cells and in cells isolated from the abdominal plasmacytoma and spleen of mice injected with the T1165-Luc-K13^−IL6 cells.