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GSK3 and IKK promote axon degeneration following trophic factor withdrawal. DRG neurons were deprived of NGF for 24 h in the presence or absence of GSK3 inhibitors indirubin 3′-monoxime (2 μm) (A) or IKKβ shRNA lentivirus (B). Axon degeneration was quantified from phase images for all treatment groups; n = 4; *, p = 0.001 by Student's t test; scale bar = 100 μm.
